Vernet Lab

The Ecology of

Polar Phytoplankton

Dr. Maria Vernet
Integrative Oceanography Division
Room 2123, Sverdrup Hall
Phone: (858) 534-5322
Fax: (858) 822-0562
Email: mvernet@ucsd.edu

November 24, 2014 | Not Logged In (public login | local login)

§ Methods and Protocols

Primary Production

  • General Concepts and Considerations
    • General Lab Safety
    • Carbonate to Bicarbonate Conversion
    • Swipe Protocol
  • Simulated in Situ (SIS)
  • Photosynthesis vs Irradiance (PI)
    • Related literature: Platt and Jassby, Journal of Phycology, 1976
  • Simulated in Situ with Ultraviolet Light Exposure

Biomass

  • General Concepts and Considerations
    • Instrument Brochure
    • Instrument Manual
    • Instrument Calibration
    • Calibration Worksheet
  • Chlorophyll a
    • Dilution Protocol>

Particulate Organic Carbon and Nitrogen (POC/PON or CHN)

  • General Concepts and Considerations
    • Samples are processed by University of California Santa Barbara's Marine Science Institute Analytical Lab on an Exeter Analytical CEC 400HA Organic Elemental Analyzer
    • Instrument Principles of Operation
  • Sample Collection

Dissolved Inorganic Nutrients

  • Silicate, Phosphate, Nitrate, Nitrite and Ammonia
    • Related Literature: Bernhardt and Wilhelms, 1967
    • Related Literature: Armstrong et al., Deep Sea Research, 1967

HPLC Pigments

  • General Concepts and Considerations
    • Instrument Methods and Calibration Notes
    • Related Literature: Wright et al., Marine Ecology Progress Series, 1991
    • Related Literature: Zapata et al., Marine Ecology Progress Series, 2000
  • Sample Collection
  • Software Notes: Batch Processing

Seabird SBE 19 SEACAT CTD

  • General Concepts and Considerations
    • Fluorometer Calibration
    • Transmissometer Calibration
    • Related Literature: Smith and Baker, Applied Optics, 1981
    • Related Literature: Pope and Fry, Applied Optics, 1997
  • Field Deployment

Grazing and Carbon Cycling Experiments

  • Microzooplankton Grazing
    • Related Literature: Landry and Hassett, Marine Biology, 1982
    • Related Literature: Gallegos, Marine Ecology Progress Series, 1989
  • DOC
    • Related Literature: Moran et al., Marine Ecology Progress Series, 2001
    • Related Literature: Moran and Estrada, Deep Sea Research Series Part II, 2002

Sample Preservation / Microscopy

  • Picoplankton Preservation
  • Flow Cytometery
  • TEP
  • Qualitative Microscopy
  • Nikon E800 User Manual

Spectrophotometers

  • Ocean Optics
  • Perken Elmer

Absorption

  • General Concepts and Considerations
    • Related Literature: Mitchell et al., NASA / Ocean Optics Protocols, 2000
  • Ap/Ad Readings
  • As Readings
  • Other Miscellaneous Notes

    • F/2 Media
    • d18O Isotope
      • Related Literature: Meredith et al., Journal of Geophysical Research, 1999