§ Methods and Protocols

Primary Production

• General Concepts and Considerations
• General Lab Safety

• Carbonate to Bicarbonate Conversion

• Swipe Protocol

• Simulated in Situ (SIS)

• Photosynthesis vs Irradiance (PI)

• Related literature: Platt and Jassby, Journal of Phycology, 1976

• Simulated in Situ with Ultraviolet Light Exposure

Biomass

• General Concepts and Considerations
• Instrument Brochure

• Instrument Manual

• Instrument Calibration

• Calibration Worksheet

• Chlorophyll a

• Dilution Protocol>

Particulate Organic Carbon and Nitrogen (POC/PON or CHN)

• General Concepts and Considerations
• Samples are processed by University of California Santa Barbara's Marine Science Institute Analytical Lab on an Exeter Analytical CEC 400HA Organic Elemental Analyzer
• Instrument Principles of Operation

• Sample Collection

Dissolved Inorganic Nutrients

• Silicate, Phosphate, Nitrate, Nitrite and Ammonia

• Related Literature: Bernhardt and Wilhelms, 1967

• Related Literature: Armstrong et al., Deep Sea Research, 1967

HPLC Pigments

• General Concepts and Considerations
• Instrument Methods and Calibration Notes

• Related Literature: Wright et al., Marine Ecology Progress Series, 1991

• Related Literature: Zapata et al., Marine Ecology Progress Series, 2000

• Sample Collection

• Software Notes: Batch Processing

Seabird SBE 19 SEACAT CTD

• General Concepts and Considerations
• Fluorometer Calibration

• Transmissometer Calibration

• Related Literature: Smith and Baker, Applied Optics, 1981

• Related Literature: Pope and Fry, Applied Optics, 1997

• Field Deployment

Grazing and Carbon Cycling Experiments

• Microzooplankton Grazing

• Related Literature: Landry and Hassett, Marine Biology, 1982

• Related Literature: Gallegos, Marine Ecology Progress Series, 1989

• DOC

• Related Literature: Moran et al., Marine Ecology Progress Series, 2001

• Related Literature: Moran and Estrada, Deep Sea Research Series Part II, 2002

Sample Preservation / Microscopy

• Picoplankton Preservation

• Flow Cytometery

• TEP

• Qualitative Microscopy

• Nikon E800 User Manual

Spectrophotometers

• Ocean Optics

• Perken Elmer

Absorption

• General Concepts and Considerations
• Related Literature: Mitchell et al., NASA / Ocean Optics Protocols, 2000

• A_p/A_d Readings

• A_s Readings


Other Miscellaneous Notes

• F/2 Media

• d¹⁸O Isotope
• Related Literature: Meredith et al., Journal of Geophysical Research, 1999